5 Remarkable Studies Proving Olive Oil (EVOO) Increases Testosterone

5 Remarkable Studies Proving Olive Oil (EVOO) Increases Testosterone

If you’ve ever wondered:

  • Does Extra Virgin Olive Oil (EVOO) Increase Testosterone Levels?
  • Can olive oil increase the size of my testicles?
  • Does olive oil increase sperm count?
  • How much EVOO do I need to consume each day?

Then you need to read this article.

Let me put it another way. This is your ultimate guide to EVOO as it relates to testosterone.

So if you want to know how extra virgin olive oil will give you:

Higher T levels.

Bigger balls.

A higher sperm count.

Then read on and find out exactly how much EVOO you need to eat.

Let’s dive right in.

The Olive Oil - Testosterone Connection

Here’s a breif overview.

Study One - A 64% Boost

We all know that olive oil has been proven to have a long list of health benefits. What most people don’t know is that this stuff is liquid gold when it comes to boosting test levels.

Let me show you what I mean:

This particular study is quite amazing.

Researchers looked at how adding olive oil to the diet of rats affected them when they were being fed a diet of genetically modified soy.

Yes I know -

The study was in rats, but check out these results:


The rats were broken into 4 groups.

  • Group 1: The control group - i.e. fed a “normal” rat diet with neither genetically modified soy or EVOO.
  • Group 2: Fed a diet of 30% EVOO.
  • Group 3: Fed a diet of 15% GMO soybean.
  • Group 4: Had both 30% olive oil AS WELL AS 15% GMO soy.

After 65 days of being fed this diet, they tested T levels of each group. This is what they found:

  • Group 1: 2.44 ng/ml
  • Group 2: 3.82 ng/ml
  • Group 3: 1.03 ng/ml
  • Group 4: 3.27 ng/ml

Let’s take a closer look at what these numbers mean.

When comparing the “normal diet” group to the group that made no other changes other than adding olive oil, levels increased by 64%!

That’s huge.

What’s even more interesting is that T levels of the group that ate genetically modified soy group plus EVOO were actually higher than the control too. That means that rats eating food that has been proven to significantly lower male hormones, but then adding EVOO to this diet actually resulted in a boost in test HIGHER than even rats eating a standard diet.

Study Two: Let’s See Some Human Proof

Okay, enough of the rats.

Let’s look at some human studies:

Specifically, 60 healthy men aged 23 to 40 years old had some baseline levels drawn:

  • testosterone
  • luteinizing hormone
  • DHEA

After ONLY 3 Weeks of adding extra virgin olive oil to their diets, something crazy happened.

Not only did testosterone increase by almost 18%, but Luteinizing Hormone (LH) also increased by almost 43%.

For those who don’t know, LH boosts the production of T in Leydig cells. This boost helps to stimulate sperm production and helps to make male characteristics more prominent. You know, things like facial hair and a deep voice.

What happened to DHEA?

Not much, but I’m good with that.


Well, not only can DHEA act as an anti androgen, but has also been shown to increase estrogen levels.

No thanks.

What are your thoughts on this human study?

Study Three: EVOO - Shows “Significantly Higher Levels of Testosterone”

Forty Wistar rats were broken into 4 groups - 10 in each group.

Each group was fed the same diet with one exception:

The fat source.

They were given either soybean, coconut, olive, or grapeseed oil.

They were fed this diet for 60 days and then their testicles were tested for several different factors including:

  • Lipid content
  • Fatty acid composition
  • Free radical biomarkers
  • Antioxidant levels
  • Steroidogenic enzymes
  • And of course hormones

This is what they found -

Animals fed with the olive oil and coconut oil diets showed the highest testicular levels of antioxidants in addition to significantly high levels of testosterone…

I’m noticing a trend here.

Let’s look at another study:

Study 4: Olive Oil Makes Your Balls Bigger

It’s pretty clear that the more olive oil you eat, the more testosterone your body makes. See the previous three studies for proof of that.


More olive oil = more test.

But, does more olive oil also increase testicle size?

Little Egg Big Egg

Let’s find out.

Scientists did an experiment at Instituto de Investigaciones Bioquimicas de La Plata:

Male Rats were broken into 4 groups. For 60 days the rats were fed either 70 grams of soy oil, olive oil, grapeseed oil, or coconut oil.

Guess what they found?

Not only did olive oil correlate with higher T levels, but it also correlated with bigger balls

If you’re anything like me, you what to know a little bit more. Bigger testicles from extra virgin olive oil? Sure, but let’s find out why.

In part, the reason your junk gets bigger is due to the fact that olive oil helps the testes to take in more cholesterol.

I thought cholesterol was a bad thing?

Not so fast.

When it comes to higher levels of testosterone, Not only is cholesterol not a bad thing. i.e. not only is it a good thing -

It’s imperative.

Testosterone would be impossible to produce (let alone boost) without a little help from our friend cholesterol.

Time to start cooking up some egg yolks.

By the way, it’s the Leydig cells in the testicles that make testosterone from cholesterol.

Starting to see how it all fits together?

Let’s recap.

More olive oil = more testosterone = bigger testicles

Study 5: Improve Sperm Quality With Olive Oil

Trying to get your girl pregnant?

Sperm quality increases significantly when olive oil is on board.

In fact:

Boars fed on squalene at 20 or 40 mg/kg/day for 60 days had a higher semen volume and higher sperm motility, which increased their reproductive performance

So more sperm volume and better in bed?

Um, yes, please.

Here’s another bonus study about oleuropein (one of the main active ingredients in olive oil):

Rats fed a high-protein diet supplemented with oleuropein at 0.1 g/100 g had higher levels of luteinizing hormone and testicular testosterone, which positively affects spermatogenesis and hence semen quality.

Most of us interested in improving our natural androgen levels are probably already eating a diet around 0.8-1.2 grams of protein per lb of body weight.

Why not add some oleuropein from olive oil and reap the benefits of better sperm quality as well?

Which leads us to:

How Much Olive Oil Should I Eat Each Day For Higher T Levels?

The only problem with excessive olive oil consumption is extra calories. According to Choose My Plate Dot Gov, 1-2 tablespoons is more than enough.

But how much then?

Well, let’s review the 5 studies from above.

The first study had rats eating an unreasonable amount each day. 30% of their diet. Granted, this study did show the biggest increase of all the studies, but still, 30% is not realistic.

The next study (the human study) had participants consume 25g per day of olive oil. A little bit more realistic. Actually, the American Heart Association recommends eating olive oil around this amount each day as well.

Studies 3 and 4:

Both of these studies were done with 20% of the diet coming from olive oil. This is again unrealistic.

One showed “a significant increase in testosterone levels” and the other showed a boost as well as bigger balls.

And last of all was our sperm study:

This study had boars eating 20–40 mg/kg/day for 60 days which increased testosterone levels and improved their semen quality and as well as reproductive outcomes.

To put this into context, a 180lb male would have to eat 1.6g - 3.2g of olive oil per day to meet these requirements. That’s really not that much, to be honest. That’s less than one tablespoon.

So, how much EVOO do you need to eat per day to increase testosterone?

Realistically, somewhere between one to two tablespoons.

That’s it.

Do you eat olive oil each day?

Do you think it helps to increase testosterone?

Leave your thoughts in the comments.

David Becker, RN, CCRN
David Becker, RN, CCRN Mr. Becker is a father, husband, and CCRN in Trauma ICU. You can read his inspiring comeback story From 412ng/dl To 923ng/dl In 6 Months - Without TRT. Feel free to send David a message here.
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